A) The biodistribution of GNS-N/C 4, GNS-COOH, and GNS-NH2 in orthotopic mouse model of breast cancer (three mice per group). B) The change in temperature of tumors upon 808 nm laser irradiation (1 W cm−2, 5 min) 24 h post intravenous injection of PBS, GNS-NH2, GNS-COOH, or GNS-N/C 4. C) Representative thermal images of mice at the end of laser irradiation. The average starting body temperature was 33.0 °C. D) Change of relative tumor volume after 808 nm laser irradiation at 1 W cm−2 for 5 min. Data are means ± s.d. (6 mice per group). E) H&E staining of tumor histological sections of different groups at six days after laser irradiation. Scale bar: 25 μm.