Figure 2.

Gut microbiota-mediated epigenetic changes are sensitive to diet. (A) Experimental design: 1. GF and ConvR mice on either chow or a HF/HS (“Westernized”) diet. 2-5. Tissues prepared and analyzed as described in Figure 1. (B) Weights of GF and ConvR mice fed a HF/HS diet at the time of sacrifice. (C) Hepatic total cholesterol, and (D) hepatic triglycerides in GF, ConvD, and ConvR mice on chow and HF/HS diets. (E) SCFA measurement in cecal contents from GF, ConvR, and ConvD animals on chow and HF/HS diet. *p<0.05, **p<0.01, error bars represent standard deviation, n = 4 mice per condition. (F-H) H4 (K5, K8, K12, and K16) acetylation in colonized liver (F), adipose tissue (G), and proximal colon (H) relative to GF controls (fold change, log₂). (I-J) H3 K18 and K23 methylation and acetylation in colonized liver (I) and proximal colon (J) relative to GF controls (fold change, log₂). *p<0.05, **p<0.01, ***p<0.001, error bars represent standard error from the mean, n=4 mice per condition