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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Cell. 2016 Nov 23;64(5):982–992. doi: 10.1016/j.molcel.2016.10.025

Figure 4.

Figure 4

SCFA-supplementation mimics colonization-induced epigenetic programming. (A) Experimental design: 1. Germ-free mice supplemented with SCFAs (GF+SCFA) or colonized (ConvD) and tissues were harvested. 2-5. Histone extracts prepared as described in figure 1. (B) Hierarchical clustering of histone PTMs in colonized and GF+SCFA mouse tissues (fold change vs. GF, log2). (C-D) Pearson's correlation of ConvD and GF+SCFA mouse tissue histone PTM states in liver (C) and proximal colon (D). (E) K-means clustering of differentially expressed hepatic genes in ConvD and GF+SCFA mice. FDR cutoff for differential expression = 0.05, n = 3 mice per condition. (F) GO-term enrichment in clusters b and c. (G) Overlap of differentially expressed genes between ConvD and GF+SCFA mice.