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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Eur J Immunol. 2016 Jun;46(6):1326–1334. doi: 10.1002/eji.201545955

Figure 2.

Figure 2

Chaperone-mediated autophagy in T-cells. In CD4+ T-cells, TCR engagement induces ROS generation, which in turn leads to the activation of NFAT. NFAT induces expression of the lysosomal protein LAMP-2A. Recognition of exposed KFERQ motifs in cytosolic proteins such as Itch and Rcan1 by an Hsc70-containg chaperone complex translocates those proteins through the lysosomal membrane. Interaction of the Hsc70-substrate complex with LAMP-2A induces the formation of a multimeric translocation complex that, with the help of an intralysosomal form of Hsc70, will unfold and transport the substrate to the lysosomal lumen, where it is degraded by lysosomal proteases.