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. 2017 Jan 12;7:40418. doi: 10.1038/srep40418

Figure 1. Accelerated phagosome maturation and enhanced bactericidal activity in BLP-tolerised macrophages.

Figure 1

(A,B) Uptake (A) and phagocytosis (B) of S. aureus and S. typhimurium (S. typhi) at 30 min by naive and BLP-tolerised macrophages were expressed as mean channel fluorescence (MCF) per cell. (C) Intracellular killing of ingested S. aureus and S. typhi at 60 min by naive and BLP-tolerised macrophages. (D,E) Phagosomal pH in naive and BLP-tolerised macrophages after chased with fluorescent probe-coupled S. aureus (D) or S. typhi (E). (F) Phagolysosome fusion in phagosomes from naive and BLP-tolerised macrophages after chased with S. aureus or S. typhi was expressed as mean fluorescence intensity (MFI). (G,H) Naive and BLP-tolerised macrophages were loaded with LysoTraker red and further incubated with S. aureus-FITC (G) or E. coli-FITC (H). Cell nuclei were stained with DAPI. Fluorescent micrographs were taken at 60 min after incubation with either S. aureus-FITC or E. coli-FITC. Scale bar = 10 μm. All Data are mean ± SD. Data in (A,B,D,E and F) are from four to six independent experiments in duplicate and data in C are from five independent experiments in triplicate. *p < 0.05, **p < 0.01 compared with naive macrophages.