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. 2017 Jan 12;7:40418. doi: 10.1038/srep40418

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Copyright © 2017, The Author(s)

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(A,B) Murine BMMs were transfected with two Rab10 specific siRNA, siRab10-1 and siRab10-2, or its scrambled siRNA (scrRNA). Expression of Rab10 mRNA (A) and protein (B) was assessed by quantitative real-time RT-PCR and Western blot analysis 24 hrs after transfection. (C,D) Phagocytosis of S. typhimurium (C) and intracellular killing of the ingested S. typhimurium (D) by BLP-tolerised BMMs transfected with either scrRNA or siRab10-2. Data are mean ± SD from four to six independent experiments in duplicate or triplicate. *p < 0.05, **p < 0.01 compared with BLP-tolerised BMMs transfected with scrRNA.