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. 2017 Jan 12;7:40418. doi: 10.1038/srep40418

Figure 5. Inhibition of NF-κB activation impairs phagosome maturation and bactericidal activity in BLP-tolerised macrophages.

Figure 5

(A,B) Naive and BLP-tolerised macrophages pretreated with two NF-κB inhibitors SN50 (25, 50 μg/ml), SC-514 (25, 50 nM) or culture medium (CM) for 60 min were stimulated with S. aureus or S. typhimurium to assess intracellular killing of the ingested S. aureus (A) and S. typhimurium (B). Data are mean ± SD from five independent experiments in triplicate. *p < 0.05, **p < 0.01 compared with naive or BLP-tolerised macrophages pretreated with CM. (C) Naive and BLP-tolerised macrophages pretreated with SN50 (50 μg/ml) or SC514 (50 nM) were loaded with LysoTraker red and further incubated with E. coli-FITC. Cell nuclei were stained with DAPI. Fluorescent micrographs were taken at 60 min after incubation with E. coli-FITC. Scale bar = 10 μm.