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. 2017 Jan 12;7:40418. doi: 10.1038/srep40418

Figure 6. Inhibition of NF-κB activation attenuates Acp5 and Rab10 expression in BLP-tolerised macrophages.

Figure 6

Naive and BLP-tolerised BMMs pretreated with the NF-κB inhibitors SC-514 (50 nM) or culture medium (CM) for 60 min were stimulated with S. typhimurium (S. typhi) for the indicated time periods. (A) Expression of Acp5 and Rab10 mRNA was assessed by quantitative real-time RT-PCR. Data are mean ± SD from four to five independent experiments and each experiment was conducted in duplicate. **p < 0.01 compared with naive or BLP-tolerised BMMs pretreated with CM. (B) Expression of Acp5 and Rab10 protein was assessed by Western blot analysis. Results shown represent one experiment from a total of three separate experiments. (C) The relative intensity of Acp5 and Rab10 was analysed by densitometry. *p < 0.05 compared with BLP-tolerised BMMs pretreated with CM.