Figure 5.

Direct interaction of polη with Rad18. (A) UV-induced colocalization of Rad18 with eGFP-polη in GM637 cells. Cells transfected with eGFP-polη and FLAG-Rad18 plasmids were irradiated at 15 J/m² and incubated for 6 h. After fixation, cells were stained for Rad18 with an antibody against FLAG. Bar=10 μm. (B) Interaction of Rad18 with polη. HA-polη was transiently expressed in GM637 cells. Immunoprecipitation was performed at various times after UV irradiation (12.5 J/m²). As a control, UV-irradiated cell lysates (6 h) were immunoprecipitated with control IgG. (C) Direct binding of Rad18 with polη. Recombinant Rad18 and polη were purified from insect cells. After incubation of the mixture, Rad18 was immunoprecipitated and polη bound to Rad18 was detected by Western blot. Polδ was used as a control.