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. 2004 Sep 9;23(19):3735–3746. doi: 10.1038/sj.emboj.7600389

Figure 2.

Figure 2

A temperature-sensitive yeast mutant of Mia40 contains reduced levels of small Tim proteins. (A) WT and mia40-3 S. cerevisiae cells were subjected to serial 10-fold dilutions, plated on YPD or YPG medium and incubated for 3 days at the temperatures indicated. (B) Mitochondria from WT and mia40-3 cells grown at 19°C were analyzed by SDS–PAGE and immunodecoration. Mia40, 15 and 45 μg mitochondrial protein, respectively; other proteins, 25 and 50 μg mitochondrial protein, respectively. (C) WT and mia40-3 cells were grown at 30 or 33°C and mitochondria were isolated. Odd-numbered lanes, 15 μg mitochondrial protein; even-numbered lanes, 45 μg mitochondrial protein. (D) Mitochondria (80 μg of protein) from cells grown at 19°C were solubilized by digitonin and subjected to BN–PAGE and immunodecoration.