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. 2017 Jan 12;7:40281. doi: 10.1038/srep40281

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Copyright © 2017, The Author(s)

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(a) Deletion of FEN1 and SUR4 genes increases chitin content in the cell wall. Cells of the parent (BY4743) and deletants were stained with CFW, and images were captured at identical conditions with a fluorescence microscope using 100× objective lens (upper panel). DIC images of corresponding fields are shown in the lower panel. (b) fen1Δ/Δ and sur4Δ/Δ strains are more sensitive to zymolyase. Zymolyase digestion of the parent and deletants was monitored by periodically measuring OD_600nm until 90 minutes. Average values of two independent experiments, carried out in triplicate each time, are shown. (c) SDS sensitivity of fen1Δ/Δ and sur4Δ/Δ strains. Cells were spotted onto synthetic complete agar plates with indicated concentration of SDS and incubated at 30 °C for 2 days before being photographed.