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. 2017 Jan 12;7:40476. doi: 10.1038/srep40476

Figure 8. Effect of extracellular vesicles on cell proliferation.

Figure 8

(a) PTr2 derived EVs promote endothelial cell proliferation. PTr2 derived EVs in different concentrations (5, 10 or 20 μg protein/mL) were added to PAOECs grown in a 6-well cell culture plate and allowed to incubate at 37 °C for 24 hrs. PTr2 derived EVs significantly increased PAOEC proliferation in a dose-dependent manner (p < 0.05, n = 5). PAOECs treated with 10 μg/mL and 20 μg/mL of PTr2 derived EVs had significantly higher proliferation compared to other treatments. (b) PTr2 cells grown in cell culture plate were treated with PAOEC derived EVs in different concentrations (5, 10 or 20 μg protein/mL) and allowed to incubate at 37 °C for 24 hrs. PAOEC derived EVs had no significant effect on PTr2 cell proliferation. Similarly, (c) PAOEC derived EVs (5, 10 or 20 μg protein/mL) were added to PAOECs grown in a culture plate and allowed to incubate at 37 °C for 24 hrs. PAOEC derived EVs did not have significant effect on PAOEC cell proliferation. Finally, (d) PTr2 derived EVs did not have significant effect on PTr2 cell proliferation after 24 hrs of incubation at 37 °C. *p < 0.05, Data is presented as mean ± SEM and derived from three independent experiments.