Figure 11.

bDLE increases the expression of CD42a⁺ megakaryocytic marker differentiation in K562 cells. (A) Untreated cells, (B) 0.07 U/ml bDLE, (C) 0.14 U/ml bDLE, (D) 0.21 U/ml bDLE, (E) 0.28 U/ml bDLE, (F) 0.35 U/ml bDLE, (G) 10 ng/ml phorbol myristate acetate or (H) dimethyl sulfoxide (1.5%, v:v) were incubated for 96 h. Cells were harvested and incubated with peridinin chlorophyll protein complex conjugated-anti-CD42a in PBS with 1% fetal bovine serum and 0.1% sodium azide for 30 min at 4°C. Samples were washed and resuspended in PBS, and 10,000 events were analyzed by flow cytometry. Flow cytometry data show representative results from one of three independent experiments. CD, cluster of differentiation; bDLE, bovine dialyzable leukocyte extract.