Figure 4.

bDLE induces arrest in S and G₂/M phases of the cell cycle in K562 cells. (A) Untreated cells, (B) 0.07 U/ml bDLE, (C) 0.14 U/ml bDLE, (D) 0.21 U/ml bDLE, (E) 0.28 U/ml bDLE, (F) 0.35 U/ml bDLE, (G) 10 ng/ml phorbol myristate acetate or (H) dimethyl sulfoxide (1.5%, v:v) were incubated for 96 h, and cell cycle progression analysis was performed using the Cycle Test™ Plus DNA Reagent kit according to the manufacturer's protocol and ModFit software. PI, propidium iodide; bDLE, bovine dialyzable leukocyte extract.