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. 2017 Jan 9;8:13946. doi: 10.1038/ncomms13946

Figure 1. DOCK8 negatively regulates IL-31 induction in CD4+ T cells.

Figure 1

(a,b) Flow cytometric analyses of thymocytes and spleen cells from 6- to 8-week-old Dock8+/− and Dock8−/− OTII Tg mice. The numerals in quadrants indicate the percentage of each subset of leukocytes. Data are expressed as mean±s.d. of 5 mice per group. **P<0.01 (two-tailed Student's t-test). See Supplementary Fig. 10 for FACS gating strategy. (c) Antigen-specific proliferation of CD4+ T cells from Dock8+/− and Dock8−/− OTII Tg mice. Data are expressed as mean±s.d. of 12 samples per group. (d) Induction of cytokine gene expression in CD4+ T cells from Dock8+/− and Dock8−/− OTII Tg mice after primary stimulation with OVA peptide. Expression (fold increase) is relative to that of unstimulated Dock8+/− samples. Data are expressed as mean±s.d. of 8 samples per group. *P<0.05 (two-tailed Student's t-test). (e) Il31 gene expression in CD4+ T cells from Dock8+/− and Dock8−/− OTII Tg mice after secondary stimulation with anti-CD3ɛ and anti-CD28 antibodies. Expression (fold increase) is relative to that of Dock8+/− samples without secondary stimulation. Data are expressed as mean±s.d. of 10 samples per group. *P<0.05; **P<0.01 (two-tailed Student's t-test). (f) ELISA showing increased IL-31 production by Dock8−/− OTII Tg CD4+ T cells after secondary stimulation. Data are mean±s.d. of 9 samples per group. *P<0.05; **P<0.01 (two-tailed Student's t-test). (g) Induction of itch in CAG-OVA mice by adoptive transfer of activated CD4+ T cells from Dock8−/−, but not Dock8+/−, OTII Tg mice. Data are expressed as mean±s.d. of four mice per group. *P<0.05 (two-tailed Mann–Whitney test).