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. 2017 Jan 12;7:40292. doi: 10.1038/srep40292

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(a,b) Time course of cleavage processing of Lys63-Ub₄ (a) and Lys48-Ub₄ (b) by 160 nM PLpro-Ubl2 and PLpro-∆Ubl2. Samples were quenched with sample buffer at the indicated time points and products were analyzed by SDS-PAGE. The negative control (time point ‘0’) was incubated without addition of enzyme.