. 2017 Jan 12;7:40292. doi: 10.1038/srep40292

Table 2. Kinetic parameters for PLpro-Ubl2 and PLpro-∆Ubl2 using three different FRET Ub-based substrates.

Enzyme	Substrate
Kinetic Parameter	RLRGG-AMC	Ub-AMC^a	ISG15-AMC^a
PLpro-Ubl2
k_cat/K_m (μM⁻¹ min⁻¹)	0.003^b	4.8 ± 0.4	13.4 ± 0.7
k_cat (min⁻¹)	N/A	20.8 ± 0.5	21.2 ± 0.3
K_m (μM)	N/A	4.4 ± 0.4	1.6 ± 0.1
PLpro-ΔUbl2
k_cat/K_m (μM⁻¹ min⁻¹)	0.003^b	4.7 ± 0.6	15.1 ± 0.8
k_cat (min⁻¹)	N/A	21.4 ± 0.8	19.0 ± 0.3
K_m (μM)	N/A	4.6 ± 0.6	1.3 ± 0.1

^aSteady-state values reported as a mean ± standard deviation, were determined from a minimum of triplicate measurements (best-fit slopes shown in Supplementary Fig. S1).

^bValue of k_app with nonsaturating substrate approximates k_cat/K_m.