Table 3.
Calculation of avidity indices from dilution series of representative samples with the software "Avidity 1.2" and with the new calculation method described in this paper.
| (a) Raw data of dilution series | ||||||
| Serum F19.3 | Serum P28 | Serum V9.2 | ||||
| Relative dilution1 | Abs (ref)2 | Abs (denat)3 | Abs (ref) | Abs (denat) | Abs (ref) | Abs (denat) |
| 1:1 | 2.765 | 0.925 | 2.668 | 1.600 | 2.936 | 1.948 |
| 1:2 | 1.962 | 0.626 | 1.833 | 1.144 | 1.943 | 1.268 |
| 1:4 | 1.428 | 0.356 | 1.300 | 0.727 | 1.269 | 0.782 |
| 1:8 | 0.831 | 0.190 | 0.824 | 0.441 | 0.778 | 0.463 |
| 1:16 | 0.592 | 0.106 | 0.480 | 0.252 | 0.409 | 0.192 |
| (b) Avidity indices obtained from different combinations of dilutions with arithmetic mean, standard deviation (SD) and coefficient of variation (CV) | ||||||
| Serum F19.3 | Serum P28 | Serum V9.2 | ||||
| Dilutions used for calculation | Avidity 1.2 (%) | new method (%) | Avidity 1.2 (%) | new method (%) | Avidity 1.2 (%) | new method (%) |
| 1:1 & 1:2 | 9.5 | 32.0 | 33.4 | 61.8 | 46.1 | 65.4 |
| 1:2 & 1:4 | 15.2 | 32.3 | 40.4 | 63.7 | 49.2 | 65.5 |
| 1:4 & 1:8 | 18.0 | 25.8 | 41.5 | 57.4 | 50.0 | 62.9 |
| 1:8 & 1:16 | 11.7 | 30.0 | 44.6 | 54.4 | 56.6 | 68.0 |
| 1:1 & 1:4 | 11.6 | 30.0 | 35.5 | 58.7 | 48.2 | 64.7 |
| 1:2 & 1:8 | 17.3 | 32.0 | 41.8 | 63.0 | 50.1 | 65.4 |
| 1:4 & 1:16 | 13.5 | 26.4 | 43.5 | 56.6 | 50.2 | 64.2 |
| 1:1 & 1:8 | 14.7 | 31.7 | 41.3 | 59.0 | 52.8 | 65.2 |
| 1:2 & 1:16 | 15.0 | 32.0 | 42.0 | 62.7 | 51.3 | 65.4 |
| Mean | 14.1 | 30.2 | 40.4 | 59.7 | 50.5 | 65.2 |
| SD | 2.8 | 2.5 | 3.7 | 3.3 | 3.0 | 1.4 |
| CV | 19.7 | 8.4 | 9.0 | 5.5 | 5.9 | 2.1 |
1Relative dilution: the starting dilutions were 1:1512 (F19.3), 1:144 (P28), 1:399168 (V9.2)
2absorbance value in EIA without denaturing washing step
3absorbance value in EIA with denaturing washing step