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. 2016 Oct 13;12(6):4419–4426. doi: 10.3892/ol.2016.5250

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — RT-qPCR analysis of miR-221/222 and PTEN expression in CAL27 and HSC6 cells transfected with miR-221/222 sponge. RT-qPCR results showed significant downregulation of miR-221/222 following transfection with miR-221/222 sponge in (A) CAL27 and (B) HSC6 cells compared with the control groups (**P<0.01). The level of PTEN expression in the miR-221/222 sponge group was significantly increased compared with that of the control and scramble groups in (C) CAL27 and (D) HSC6 cells (**P<0.01). RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR-221/222, microRNA-221/222; PTEN, phosphatase and tensin homolog.