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. 2016 Oct 4;12(6):4556–4560. doi: 10.3892/ol.2016.5219

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of OPN knockdown or over-expression on adhesion protein and GAPDH expression in the human primary renal cortical endothelial cell line. (A) The expression of pY118-paxillin, pY-FAK, total paxillin and total FAK was examined by western blot analysis. (B) Statistical analysis of protein expression from western blotting. All data are presented as the mean ± standard error of the mean of 4 individual experiments. **P<0.01. OPN, osteopontin; FAK, focal adhesion kinase; over OPN, over-expression of pcDNA3.1-OPN-green fluorescent protein; over blank, empty carrier over-expression; si-OPN, small interfering RNA interference; si-blank, small interfering RNA.