FIG 2.

Application of the 103 HRMA assay to plasmids and patient samples. −dF/dT plots of the common SNP genotypes at position 103 were obtained by using either plasmids (A to D) or patient samples (E to H) and complementary probes. The presence of a probe peak to the left of the amplicon-amplicon peak indicates successful probe binding, allowing the allocation of a 103 genotype to the sample. Shown are wild-type (AAA) plasmid (A) and wild-type (AAA) patient sample (E), wild-type (AAG) plasmid (B) and wild-type (AAG) patient sample (F), mutant (AAC) plasmid (C) and mutant (AAC) patient sample (G), and mutant (AAT) plasmid (D) and mutant (AAT) patient sample (H). The large peak on the right in panel E and the small peaks on the right in panels F to H correspond to the melting of the PCR template DNA.