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Analytical sensitivity of HRMA assays. Shown are −dF/dT plots of dilution series. (A) Mutant AAC 103 plasmid diluted in wild-type AAA plasmid. The smallest peak that can be reliably identified is at 5%. (B) Mutant TGT 181 plasmid diluted in wild-type TAT plasmid. The lower limit of detection is 5%. (C) Mutant GTG 184 plasmid diluted in wild-type ATG plasmid. The smallest peak that can be reliably identified is at 20%.
