TABLE 1.

Sequences of HRMA-PCR primers and unlabeled oligonucleotide probes

Codon	Primer		Probe
	Name	Sequence (5′-3′)	Name	Sequence (5′-3′)a
103	103-Forward	AGGAATACCACACCCAGCAG	wt103A	CTGATTTTTTCTTTT
	103-Reverse	AAATATGCATCCCCCACATCC	wt103G	CTGATTTCTTCTTTT
			mt103C	CTGATTTGTTCTTTT
			mt103T	CTGATTTATTCTTTT
181 and 184	181/4-Forward	AGCAAAAAATCCAGAAATAGTCATC	wt181	ATATTGATAGATGAC
	181/4-Reverse	CTATGTTGCCCTATTTCTAAGTC	mt181C	ATATTGACAGATGAC
			wt184	ATATTGATAGATGAC
			mt184V	ATATTGACAGATGAC

^aNucleotide base pairs with the mutant base of interest are in boldface.