TABLE 1.
Diagnostic evaluation and sequence results in STGA-D locus in 9 patients with PCR-confirmed amoebic appendicitis
| Case no. | Pathological finding on medical records | Histopathological re-examination |
E. histolytica-specific PCR | Sequence type | |
|---|---|---|---|---|---|
| PAS stain | H&E stain | ||||
| AA19 | Nonamoebic | Positive | Positive | Positive | 8SD |
| AA25 | Nonamoebic | Positive | Positive | Positive | 12SD |
| AA33 | Amoebic | Positive | Positive | Positive | 12SD |
| AA38 | Nonamoebic | Positive | Positive | Positive | 15SD |
| AA42 | Nonamoebic | Negative | Negative | Positive | 16SD |
| AA44 | Nonamoebic | Negative | Negative | Positive | 15SD |
| AA45 | Nonamoebic | Negative | Negative | Positive | 15SD |
| AA56 | Amoebic | Positive | Positive | Positive | 12SD |
| AA57a | Amoebic | Positive | Positive | Positive | 12SD/15SD |
a
Entamoeba infection was missed on initial histopathological examination with H&E staining at appendectomy but was identified on rereview of the appendix specimen with PAS and H&E staining at 14 days after surgery when the patient developed intestinal perforation.