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(A) ELISA and MagPix LLOD determination for LASV IgM detection using native antigens. (B) ELISA and MagPix LLOD determination for EBOV IgM detection using native antigens. The negative control (NC) was normal NHP serum. The cutoff for “positive” detection was defined as 2.5× the average NC absorbance (ELISA) or MFI (MagPix) values. Gray bars indicate positive detection and black bars indicate no significant difference from the NC. Error bars represent the standard deviations of n ≥ 3.
