TABLE 2.
Comparison of ELISA and MagPix experimental components for antigen detection
| Sequential order | Antigen detection |
||
|---|---|---|---|
| ELISA (buffer) | MagPix (buffer) | MagPix (serum) | |
| 1 | Plate | Microspheres | Microspheres |
| 2 | Coating antigen-specific mAb | Coating antigen-specific mAb | Coating antigen-specific mAb |
| 3 | Antigen cocktail | Antigen cocktail | Test serum |
| 4 | Detector antigen-specific mAb (biotinylated) | Detector antigen-specific mAb (biotinylated) | Detector antigen-specific mAb (biotinylated) |
| 5 | HRP-linked streptavidin | R-phycoerythrin-linked streptavidin | R-phycoerythrin-linked streptavidin |