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. 2016 Oct 27;12(6):5229–5234. doi: 10.3892/ol.2016.5324

Figure 1.

Figure 1.

Establishment of the CNE2-shANXA1 cell line with knockdown of ANXA1. (A) Western blotting was used to detect the expression levels of ANXA1 in the untransfected (control 1), empty vector pLKO.1-transfected (control 2) and pLKO.1-ANXA1-shRNA-tansfected CNE2 cells. (B) Histogram shows the expression levels of ANXA1 in the three cell lines as determined by densitometric analysis. β-actin was used as an internal loading control. *P<0.05; CNE2-shANXA1 vs. control 1 or control 2. ANXA1, Annexin A1; shRNA, small hairpin RNA.