Figure 4.

DIOS disturbs the balance between Notch3 and p53 and resulted in the inhibition of the NF-κB signaling pathway. (A) Notch3, NF-κB and p53 protein levels were determined by immunoblotting following knockdown of Notch3 by siRNA transfection. (B) Notch3, NF-κB and p53 were detected by immunoblotting following knockdown of p53 by siRNA transfection. (C) The clone formation ability of HepG2 cells was tested upon blocking Notch3, following DIOS treatment alone or subsequent to a combination of the two approaches. (D) Notch3, p53, NF-κB, Bax, Bcl-2 and PARP were detected when Notch3 was blocked with DIOS treatment. DIOS, diosmetin; NF, nuclear factor; Bcl, B-cell lymphoma; Bax, Bcl-2-like protein 4;; PARP, poly (ADP-ribose) polymerase; sh, small hairpin; si, small interfering.