Expression changes in genes and/or proteins associated with the regulation of cell cycle and cell apoptosis (epithelial bile duct marker CK19 and proliferation marker PCNA). (A) Compared with the control group, the gene expression of CK19 significantly decreased, while the gene expression of Bax, Apaf-1, caspase-9 and caspase-3 significantly increased, in the forbesione-treated group. Data are presented as the mean ± standard deviation. *P<0.05. (B and C) Representative immunohistochemical staining of Ham-1 allograft tissue (magnification, ×40). Compared with the control group, the protein expression of (B) CK19, PCNA, cyclin A and (C) Bcl-2 was decreased, while the protein expression of (C) Bax, caspase-9 and caspase-3 was increased, in the forbesione-treated group. Forb, forbesione; Apaf, apoptotic protease activating factor; Bcl, B-cell lymphoma; Bax, Bcl-2-like protein 4; CK, cytokeratin; PCNA, proliferating cell nuclear antigen.