Table I.
Primary antibodies used in western blot analysis.
| Target | Type | Catalog no. | Host species/targeted species | Dilution | Secondary antibody |
|---|---|---|---|---|---|
| β-actin | Monoclonal | A1978 | Mouse anti-human | 1:3,000 | Goat anti-mouse |
| Bcl-2 | Polyclonal | sc-492 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| Bax | Polyclonal | sc-493 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| Survivin | Monoclonal | sc-17779 | Mouse anti-human | 1:400 | Goat anti-mouse |
| Caspase-9 | Polyclonal | sc-8355 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| Procaspase-3 | Polyclonal | sc-7148 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| Activated caspase-3 | Polyclonal | #9662s | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| Fas | Polyclonal | sc-715 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| FADD | Polyclonal | sc-5559 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| Procaspase-8 | Monoclonal | #9746 | Mouse anti-human | 1:400 | Goat anti-mouse |
| Cyclin E | Polyclonal | sc-481 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| Cyclin A | Polyclonal | sc-751 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| Cdk2 | Polyclonal | sc-163 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| p21 | Polyclonal | sc-397 | Rabbit anti-human | 1:400 | Goat anti-rabbit |
| p27 | Polyclonal | sc-527 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| IκB-α | Polyclonal | sc-371 | Rabbit anti-human | 1:500 | Goat anti-rabbit |
| NF-κB/p65 | Polyclonal | sc-109 | Rabbit anti-human | 1:500 | Goat anti-rabbit |
| Caspase-12 | Polyclonal | AB3613 | Rabbit anti-human | 1:200 | Goat anti-rabbit |
| CK19 | Monoclonal | ab7754 | Mouse anti-human | 1:500 | Goat anti-mouse |
| PCNA | Monoclonal | NCL-L-PCNA | Mouse anti-human | 1:500 | Goat anti-mouse |
Bcl, B-cell lymphoma; Bax, Bcl-2-like protein 4; FADD, Fas-associated death domain; Cdk, cyclin-dependent kinase; IκB-α; inhibitor of κB-α; NF, nuclear factor; CK, cytokeratin; PCNA, proliferating cell nuclear antigen.