Table III.

Primer sequences used for reverse transcription-quantitative polymerase chain reaction.

Gene	Primer sequences	No. of cycles	Ta,°C	GenBank accession no. (ref.)
GAPDH	Forward: 5′-GGCATTGTGGAAGGGCTCAT-3′	35	60	(25)
	Reverse: 5′-GACACATTGGGGGTAGGAACAC-3′
CK19	Forward: 5′-GCGGGACAAGATTCTTGGTG-3′	40	62	NM_008471.2
	Reverse: 5′-CTTCAGGCCTTCGATCTGCAT-3
Bax	Forward: 5′-AGCTGCAGAGGATGATTGCT-3′	40	62	(25)
	Reverse: 5′-CTCTCGGAGGAAGTCCAGTG-3′
Apaf-1	Forward: 5′-ATCCTGGTGCTTTGCCTCTA-3′	50	62	(26)
	Reverse: 5′-TACACCCCCTGAAAAGCAAC-3′
Caspase-9	Forward: 5′-CTGCACTTCCTCTCAAGGCA-3	40	52	XM_005139261.1
	Reverse: 5′-GAAACAGCATTGGCGACCTG-3′
Caspase-3	Forward: 5′-TTCGAGCCACCGAGGAGATA-3′	40	54	NM_001281582.1
	Reverse: 5′-TTGGGGACATCATCCACACG-3′

Ta, annealing temperature; CK, cytokeratin; Bax, B-cell lymphoma-2-like protein 4; Apaf, apoptotic protease activating factor.