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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Methods Mol Biol. 2016;1403:363–383. doi: 10.1007/978-1-4939-3387-7_19

Table 4.

EtpA as a prototype molecule in reverse vaccinology algorithms

Program Result/prediction Notes/score
Signal-P No signal peptide identified –
PredictProtein Predicted location: secreted –
Secretome Secreted (SecP) 0.939918
Psortb Extracellular 10.00
CELLO Extracellular 2.777
Vaxign Adhesin via SPAAN [47]
Motifa Multiple domains identified:
CDD130956, adhes_NPXG
CDD214908, haemagg_act
CDD257462, ESPR		 Domain description
Filamentous hemagglutinin family N-terminal domain
Hemagglutination activity domain
Extended signal peptide of type V secretion system
a

Motif searches both CDD and Pfam databases