Figure 2.

SATB1 mRNA and protein levels in MHCC-97H (high metastatic potential) and HepG2 (low metastatic potential) liver cancer cell lines were assessed by western blot analysis and polymerase chain reaction. SATB1 (A) protein and (B) mRNA expression was higher in MHCC-97H cells than HepG2 cells. β-actin and GAPDH were used as loading controls. (C) Quantification of western blot analysis revealed that SATB1 protein expression was significantly higher in MHCC-97H cells compared with HepG2. *P<0.05. Data are presented as the mean ± standard error of the mean of three independent experiments. ATB1, special AT-rich DNA-binding protein-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.