Figure 1.

Upregulated secretion of IL-1β in gallbladder tumors and chronic cholecystitis. (A) The representative ELISA displays the comparison between the secretion of IL-1β among the tissues of gallbladder cancer, chronic cholecystitis and normal gallbladder. (B) The IL-1β concentrations were also investigated in the gallbladder cancer cell lines GBC-SD, SGC996 and the non-malignant gallbladder epithelial cell line HIBEpic. (C) Relative expression of IL-1β mRNA in GBC-SD and SGC996 compared to HIBEpic cells. The quantitative data (A and B) presented in the bar graphs were obtained from three independent ELISA experiments. Data are expressed as the mean ± standard deviation. *P<0.001 compared with normal gallbladder tissues/cells. IL, interleukin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ELISA, enzyme-linked immunosorbent assay.