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IL-1β increased expression of Twist in gallbladder cancer cell lines. (A) Changes in the Twist protein levels of cells exposed to IL-1β detected by western blotting. (B) Measurement of the Twist mRNA levels of cells treated with Twist-shRNA detected by reverse transcription-polymerase chain reaction. (C) Changes in the Twist protein levels of cells treated with Twist-shRNA detected by western blotting. IL, interleukin; shRNA, small hairpin RNA.
