Figure 3.

Total T cells were isolated from peripheral blood samples and stimulated with 10 ug/ml phytohemagglutinin. ODNs against human miRNA-155 were used as the anti-miRNA-155 inhibitor. (A) miRNA-155 expression levels were detected using reverse transcription-quantitative polymerase chain reaction analysis. Cells proliferation was evaluated using (B) cell count and (C and D) immunofluorescence staining of PCNA. Magnification, ×100. *P<0.05 vs. sseptic shock (control; n=6). AS-ODNs, antisense oligodeoxynucleotides against human miRNA-155; SE-ODNs, sense oligodeoxynucleotides complementary to human miRNA-155; PCNA, proliferating cell nuclear antigen.