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Primer sequences, annealing temperatures and restriction enzymes of two polymorphisms.
| Polymorphism | Primer sequence | Annealing temperature (°C) | Restriction enzyme |
|---|---|---|---|
| MMP-9 | F: 5′-GCCTGGCACATAGTAGGCCC-3′ | 56 | SphI |
| −1562 C>T | R: 5′-CTTCCTAGCCAGCCGGCATC-3′ | ||
| MMP-3 | F: 5′-GAT TACAGACAT GGG TCACA-3′ | 53 | XmnI |
| −1612 5A/6A | R: 5′-TTTCAATCAGGACAAGACGAAGTTT-3′ |
MMP, matrix metalloproteinase; F, forward; R, reverse.
