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. 2016 Oct 18;12(6):4850–4856. doi: 10.3892/ol.2016.5286

Figure 2.

Figure 2.

Alterations in expression of proteins involved in the EMT and phosphorylation of Akt in MEF cells expressing cyclin D1b and/or activated K-ras. (A) Immunoblot analyses of the expression of EMT proteins and the phosphorylation of Akt. (B) Effect of an Akt inhibitor on the invasiveness of MEF cells expressing cyclin D1b. The Akt inhibitor was used at a concentration of 25 µM. Each sample was assayed in triplicate, and the bar represents the mean ± standard deviation. P=0.9947, compared with untreated WT-LT MEF cells. *P=0.0116. EMT, epithelial-to-mesenchymal transition; MEF, mouse embryo fibroblast; K-ras, v-K-ras2 Kirsten rat sarcoma viral oncogene homolog; WT, wild-type; P, phosphorylated; BP, pBABE puro.