Figure 1.
Representative results of methylation-specific polymerase chain reaction analyses. Peripheral blood lymphocytes DNA treated by SssI methyltransferase was used as the methylation-positive control and DNA from normal lymphocytes was used as the unmethylation-positive control. NEG, negative control; Lane L1-L2, breast cancer samples; M, methylation; U, unmethylation; POS, positive control; BRCA1, breast cancer 1, early onset; DNA repair associated; GSTP1, glutathione S-transferase pi 1; P16INK4A, cyclin dependent kinase inhibitor 2A; MGMT, O-6-methylguanine-DNA methyltransferase; PTEN, phosphatase and tensin homolog; RARβ2, retinoic acid receptor beta 2; CCND2, cyclin D2.