Figure 3.

Adiponectin blocked the upregulating effect of CSE on 4-HNE and ROS levels in 16HBECs. 16HBECs were treated with 5% CSE, 5% CSE + HMW or gAd at different concentrations, and (A) 4-HNE was detected by ELISA and (B) ROS was detected by flow cytometry. Each bar represents the mean ± standard deviation from three samples. *P<0.05 vs. 5% CSE (−)/HMW (−)/gAD (−).CSE, cigarette smoke extract; gAd, globular domain; HMW, high molecular weight; 4-HNE, 4-hydroxy-nonenal; ROS, reactive oxygen species.