Figure 4.

Transfection and reporter assay for the effect of ADM and PTX on ATF2 internal ribosome entry segment activity. (A and B) Bel7402 cells were transfected with the pRL-ATF2-FL plasmid and treated with or without increasing concentrations (0.01, 0.2 and 0.4 µg/ml) of (A) ADM and (B) PTX. Data are represented as the mean ± standard deviation of RL and FL activities in ≥3 independent experiments. RL, Renilla luciferase; FL, firefly luciferase; ATF2, activating transcription factor 2; ADR, adriamycin; PTX, paclitaxel.