Table I.
Oligonucleotide primers used in polymerase chain reaction for plasmids construction.
| Vector (nt) | Primer sequence, 5′-3′ | Temperature, °C |
|---|---|---|
| pR-ATF2-F (31–299) | F1: GGAATTCCATATGGAAGCCTGTGGGAGCCC | 50.0 |
| R1: CGGAATTCAAGTTGAATAACTTATCACATTC | ||
| pR-ATF2-F (51–299) | F2:GGAATTCCATATGGCCTTTAAAGTGCCGTTCAG | 50.0 |
| R2: CGGAATTCAAGTTGAATAACTTATCACATTC | ||
| pR-ATF2-F (76–299) | F3: GGAATTCCATATGTCCTCCAGGGGTGCTTTGTA | 50.0 |
| R3: CGGAATTCAAGTTGAATAACTTATCACATTC | ||
| pR-ATF2-F (141–299) | F4: GGAATTCCATATGACTAGTGACCTGGAAAGGGT | 50.0 |
| R4: CGGAATTCAAGTTGAATAACTTATCACATTC | ||
| pR-ATF2-F (1–229) | F5: GGAATTCCATATGGTTGTCAGTCCGATCTCGC | 52.0 |
| R5: CGGAATTCATTTACTCTGCTTCCAGGAATACC | ||
| pR-ATF2-F (1–159) | F6: GGAATTCCATATGGTTGTCAGTCCGATCTCGC | 52.8 |
| R6: CGGAATTCCCCTTTCCAGGTCACTAGTTC | ||
| pR-ATF2-F (1–89) | F7: GGAATTCCATATGGTTGTCAGTCCGATCTCGCG | 54.5 |
| R7: CGGAATTCGCACCCCTGGAGGAAAAGG |
F, forward; R, reverse; ATF2, activating transcription factor 2.