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. 2016 Oct 13;12(6):4589–4597. doi: 10.3892/ol.2016.5249

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — (A) Bcl-2 and Bcl-xl expression was measured by reverse transcription-quantitative polymerase chain reaction. *P<0.05. (B) Western blot analysis revealed similar results. (C) RKO cells were co-transfected with miR-217 and MAPK1 overexpression plasmid or with a control, and the absorbance (representative of MTT reduction) was measured at 1, 2, 3, 4 or 5 days after transfection. *P<0.05 compared with the control groups. (D) Representative results of colony formation assay in RKO cells co-transfected with miR-217 precursor and MAPK1. *P<0.05 compared with the control groups. (E) Apoptosis assay of RKO cells transfected with miR-217 precursor and MAPK1 overexpression plasmid or with a control. *P<0.05 compared with the control groups. (F) MAPK1, Bcl-2 and Bcl-xl protein expression was measured in RKO cells co-transfected with miR-217 precursor and MAPK1 overexpression plasmid or with a control. mRNA, messenger RNA; NC, negative control; miR, microRNA; Bcl, B-cell lymphoma; xl, extra large; MAPK, mitogen-activated protein kinase.