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. 2016 Nov 11;12(6):3837–3842. doi: 10.3892/etm.2016.3893

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Copyright: © Tang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-218 inhibits the proliferation and migration of gastric cancer cells via regulating the expression of Ang-2. (A) Representative images (magnification, ×200) and quantification of the in vitro invasion assay for NCI-87 and HGC-27 cells transfected with pre-miR-218 or scramble using Transwell assay. Scale bar, 50 µm. (B) Results of the soft agar colony formation assay. (C) Reverse transcription-quantitative polymerase chain reaction analysis of miR-218 expression levels in transfected cells. (D) ELISA detection of Ang-2 protein levels in the culture supernatant. (E) Western blot of the protein expression levels of Ang-2 in gastric cancer cells. (F) Ang-2 protein levels determined by western blot image analysis. Results are presented as the mean ± standard error of the mean of three independent experiments. ***P<0.001. miR, miRNA; Ang-2, angiopoietin-2.