Table I.
Reverse transcription quantitative PCR reaction conditions.
| Condition | Initial denaturation | Amplification stage | Melt curve | |
|---|---|---|---|---|
| Cycle number | 1 | 40 | 1 | |
| Temperature, °C | 95 | 95 | 60 | 95 |
| Time, sec | 30 | 5 | 30 | 30 |
PCR, polymerase chain reaction.
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