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. 2016 Nov 15;12(6):3963–3971. doi: 10.3892/etm.2016.3902

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Copyright: © Ji et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Adipogenic differentiation of sheep metanephric mesenchymal stem cells (MMSCs). (A) As a negative control, cells cultured in complete medium showed no changes in morphology and were negative for Oil Red O staining. (B) After induction for 12 days, MMSCs became fibroblast-like to oblate and formed numerous intracellular lipid droplets. Lipid droplets were stained with Oil Red O (scale bar, 100 µm). (C) Expression of adipocyte-specific genes LPL and PPARG were detected by reverse transcription-quantitative polymerase chain reaction in the induced group after induction for 12 days. Adipocyte-specific genes were not expressed in the control group. Lane 1: GAPDH served as the internal control in the inducted group; lane 2: PPARG was positive in the inducted group; lane 3: LPL was positive in the inducted group; lane 4: GAPDH served as the internal control in the control group; lane 5: PPARG was negative in the control group; lane 6: LPL was negative in the control group.