Skip to main content
. 2016 Oct 5;12(6):4779–4784. doi: 10.3892/ol.2016.5229

Figure 2.

Figure 2.

Analysis of transfection efficiency and miR-193a-3p expression levels by fluorescence microscopy and reverse transcription-quantitative polymerase chain reaction. (A) The transfection efficiency was 85 and 80% in Eca109 and TE-1 cells, respectively (magnification, ×200). (a) Eca109 cells transfected with Fam-labeled negative control. (b) Eca109 cells exhibiting green fluorescence 6 h after transfection with Fam-labeled negative control. (c) TE-1 cells transfected with Fam-labeled negative control. (d) TE-1 cells exhibiting green fluorescence 6 h after transfection with Fam-labeled negative control. (B) Fold-changes in miR-193a-3p expression in Eca109 and TE-1 cells treated with miR-193a-3p inhibitor or negative control were analyzed 24 h after transfection. Data are presented as the mean ± standard deviation. **P<0.01 vs. the negative control. All experiments were performed in triplicate. miR, microRNA; Fam, fluorescein amidite.