Table I.
Primers used for MS-HRM assay.
| Amplified region | ||||||
|---|---|---|---|---|---|---|
| Primer | Sequences (5′-3′) | Ta°C | Product size (bp) | Number of GC dinucleotide | Accession no. | Nucleotide numbers |
| SEPT9 (1st location) | F: CGGTGATAGAGAATTTTGTTTGGT | 60 | 178 | 11 | NC_000017.11 | 77372911–77373089 |
| R: CGACCTCAACCCCTCCC | ||||||
| SEPT9 (2nd location) | F: GACGTGTTGGAGAGGATTTTG | 60 | 181 | 24 | NC_000017.11 | 77373582–77373763 |
| R: CGAATACCCCTAACAAAATCCC | ||||||
| NTRK3 | F: TGGTTCGGGAGATGTTTTT | 58 | 164 | 16 | NC_000015.10 | 88255602–88255765 |
| R: AAACGAACCAACAACTAATTAAA | ||||||
Ta, appropriate annealing temperature. MS-HRM, methylation specific-high resolution melting.