Table 2. Ranking and statistical analyses of candidate reference genes using BestKeeper, geNorm and NormFinder.
| BestKeeper | geNorm | NormFinder | |||||
|---|---|---|---|---|---|---|---|
| Ranking | r | Ranking | SD | Ranking | M | Ranking | SV |
| rps18 | 0.992 | actb1 | 1.027 | tbp | 1.006 | tbp | 0.069 |
| tbp | 0.987 | tbp | 1.099 | rps18 | 1.023 | rps18 | 0.113 |
| rps11 | 0.983 | ef1a | 1.160 | rps11 | 1.030 | actb1 | 0.141 |
| actb1 | 0.983 | rps11 | 1.217 | actb1 | 1.035 | rps11 | 0.190 |
| ef1a | 0.777 | rps18 | 1.297 | ef1a | 1.470 | ef1a | 0.622 |
| hprt1 | 0.718 | gapdh | 1.761 | hprt1 | 2.285 | hprt1 | 1.191 |
| gapdh | 0.493 | hprt1 | 2.386 | gapdh | 2.352 | gapdh | 1.252 |
Notes.
Abbreviations
- SD
- Standard deviation
- r
- Pearson product-moment correlation coefficient
- SV
- stability value
- M
- M value of stability