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Effect of combined treatment with TEM and CCM on apoptotic proteins. (A) Caki-1 and (B) OS-RC-2 cells were treated with TEM alone, CCM alone, TEM and CCM, or the Ctr for 48 h. PARP and caspase 3 protein expression levels were determined in the two cell lines using western blot analysis (*P<0.05 vs. Ctr). TEM, temsirolimus; CCM, curcumin; Ctr, control; PARP, poly ADP-ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
